Thursday, May 30, 2013

2013 has been interesting year for Cuatro cienegas


May 2013

Some very interesting weeks for the future of agriculture in the desert of Coahuila just ended. The state government, in coordination with the National Water Commission and the National Commission of Protected Areas, closed several illegal wells in Cuatro Ciénegas, and are already making the "inventory" of legal users and putting gauges to know how much water they use, and how much they have to use.
On the other hand, two agronomists from Beta Santa Mónica (Chava Ordaz y Edgar Ramirez) arrived to my laboratory on Monday. Martín Carrillo, who is working with us as the leader of the project of environmental education in the CBTA22, and Nadia Casanova, the teacher that coordinates the Biology courses in the CBTA, their mission: to learn how to take DNA from soil, bacteria and fish, and find molecular markers to solve specific problems. What kind of problems?
1. We discovered that neither the CBTA’s alfalfa nor the one that Beta uses (both in el Hundido and in Torreon) are establishing nitrogen-fixing nodules. This doesn’t surprise me from the CBTA’s alfalfas, but the ones from Torreon, and from El Hundido where a small fortune is spend to buy an inoculum of Rhizobium meliloti, the symbiotic of alfalfa, did.  
For those who don’t know anything about the subject, a good part of the leguminous plants are capable, from millions of years ago, of establishing ‘a little house with food and protection’ for soil-bacteria of the Rhizobiaceae family; the ‘house’ is a structure in the root that is called nodule. This evolutionary courtesy is not for free, in exchange of housing and food, the bacteria that live inside the nodule work like slaves to break the triple bond that joins the dinitrogen molecule (N2) making this gas, so abundant in the atmosphere, to become ammonium NH3, which is an available form of N for life. In a natural way this works if the ‘conversation’ between plant and bacteria is formed by legitimate molecular signals. Therefore the beans recognize the Rhizobium etli, its particular symbiont, as native from Mexico as the bean, the alfalfa that is native from Turkey recognizes the Rhizobium meliloti, and therefore, outside Turkey and Southern Europe, the alfalfas have to be artificially inoculated. The problem is that the Mexican soil is so rich in its own bacteria that the foreign ones can’t survive, the agronomists didn’t know this; they thought that everything was in order. 

2. To be able to know if there is a natural capacity to set nitrogen, you have to go directly to the source, that is, searching for the enzyme responsible of breaking the triple bond of N2. This is called nitrogenase and is encoded by several genes, one of them is called nifH, and is the one that we will look for directly on the floor of CBTA, Torreon, and El Hundido, using  isolated colonies from the soil around the root or directly in the total DNA on the ground…If the molecular biology laboratory of the CBTA22 can assemble these techniques, they could check not only their soil capacities, but also the capacities of the farmers in the region, and this way the students learn, provide a service to the community, and that service pays them off!!!

3. Chava, Edgar and Martín, along with the students of the CBTA22 are going to set up experiments with beans and other leguminous plants to see what kind of leguminous can in fact nodule and fix nitrogen in the soil of the area.

4. From the biodiversity of the microbial mats, my laboratory is going to look for inoculums that simultaneously optimize the soil and plant health, as well as developing molecular markers that the CBTA22 will use to determine healthy farming practices. In exchange, the engineers compromised themselves to support the productivity of the CBTA22, to sanitize their well, to fix the greenhouse, and to bond with the students. We want the students of the CBTA to be the best prepared in the region to address the XXI century agriculture with sustainable practices directed by scientific knowledge.


Monday, March 11, 2013

Cuatrociénegas in March 2013: There is still hope even at the edge of the cliff.

GOOD NEWS!!!

On Monday March 5, we arrived to Cuatro Ciénegas (CCC) to a reunion with the agronomists from Beta Santa Monica and Gustavo Diaz de Leon. The meeting’s idea was to demonstrate the progress that Beta Santa Monica has been done about the water saving in the ranch of Tanque Nuevo in the Hundido valley south of CCC. A few months ago, I asked him to change the variety of alfalfa that he uses, to one that tolerates drought and that requires less water. I also ask him to irrigate at night to reduce evaporation.

Guess what?? Gustavo listened to us, and sent his experts to find more ecological solutions that meet our requirements. First, they went to an agrarian university in Jalisco, that specializes in ecotechniques, to see how to save water, and then they went to the US to inquire about a drought-resistant alfalfa. What they commented at the meeting is that the most difficult step to take was to accept that the mentality has to change, and that something new has to be done to allow, in the future, the sustainability of the CCC oasis.
They now irrigate at night (it was difficult to change the people’s shifts, and to watch how to monitor the damages at night) a variety of alfalfa from Nuevo Mexico that is irrigated only once every third day, and in just a few months several millions of liters of water were saved!!! They now think that their friends from Torreon have to do the same, since the aquifer of this place is losing more than one meter a year!!!

Another thing that we did during this trip to CCC, was to go with those from Beta to the CBTA 22, and by talking with the principal, Thelma, we agreed that in exchanged of the CBTA’s laboratory, she and her students will revise the nitrogen fixators in the alfalfa.  Those from Beta are going to support the CBTa with the new greenhouse that the SEP installed them…but that [SEP] didn’t explain to them how to get it started…ad hoc, Beta Santa Monica is going to pay for the salary for the two technicians supporting the CBTa.
The first one will be trained, by us, in molecular techniques so the students can have a service lab that that will produce a higher yield at the same time that it will educate them, and they will have the big plus that will allowed them to graduate with a specialty in molecular markers for agricultural technicians (to detect by PCR and sequences, the health of crops and animals).
The second one is going to be an expert in greenhouses, and is going to help them grow cornfields and organic vegetables by drip irrigation, and in a protected area, parents will be able to see in a tangible way how this type of agriculture is more profitable than irrigating by flooding the alfalfa crops.

Next week, Thelma and Martin Carrillo (management expert who we were lucky enough to hire for the project) will go to Torreon to see how do they handle the greenhouses, and on April, Martin and the agronomists will come to our laboratory at the UNAM to see what kind of agricultural problems they have, and how can we help setting up diagnostic techniques that they can implement in the CBTa’s lab. This will close a virtuous circle that will not only change the way industrialists from Torreon see the water but also benefit themselves by helping to educate the community of CCC through their youth.

At night we saw Arturo Gonzalez, from the desert museum, and his team of paleontologists. He told us that the government of Coahuila finally closed the purchase of La Becerra, and that they want our help to make a site museum.  On the other hand, they told us that PRONATURA had bought the right over 250,000 cubic meters of water a year for the recharge of the Garabatal, and with all that, they hoped to at least partially restore the wetland.

On Tuesday, march 5, we went with a group of students from the CBTa 22, my PhD student, Mirna, and the people from CINVESTAV to Pozas Rojas to collect. I was impressed by how much the young people from the CBTa have changed in just a few months of being interns and how they have a much better idea of what ecology is. After that, we went to the CBTa’s lab to isolate the collected microbes, and again I was surprise by how well the students learned what Manuel Rosas taught them in November and December of 2012. Now follows that they will teach to Martin Carillo how to extract and amplify DNA.

Wednesday March 7, along with the teachers of CONCENTRARTE, I gave a little talk to the kindergartners about what a scientist does in CCC, and why the water from this place is so special. Then, changing the setting, Gabriela Olmedo from the CINVESTAV explains to the students of the CBTa, what a sequence means, and how to decipher the code; he made a tree WITH THE SEQUENCES OBTAINED BY THE STUDENTS OF THE CBTa!!! This is the first effort in Mexico to make high school level ecology. It was very, very exciting. What follows are not only the samples of Pozas Rojas, which they began to process, but the comparison with the bacteria from the water they drink or irrigate their crops; this way they will compare what is “native” to the oasis, with what is associated to us humans. The results are going to be presented at the 3rd congress of Biochemistry and Bacteria’s Molecular Biology which will be held at CCC on October of this year.

After that, we went to Churince and there was water!!! In just 3 weeks that the Garabatal was recharged, the Churince raised its level!!! And we saw 3 turtles swimming…not too much fish.
THERE IS STILL HOPE EVEN ON THE EDGE OF THE CLIFF.

Valeria Souza

Saturday, February 9, 2013
International Wetlands day.

Thursday January 30, I flew to Saltillo to reunite with the team of social communication from SEMARNAT, CAN, CONANP, PROFEPA and invited journalists.
On the airport I had to give them a small press conference to explain them what does Cuatro Ciénegas means to science. In the route to Cuatro Ciénegas, the journalists and I continued talking. We got there by noon, tired but enthusiastic to see more. Juan Carlos Ibarra, the person in charge of the protected area, took us to la Becerra and explained what does a wetland means; then he took us to the ejido of Cuatro Ciénegas to see both the threat (alfalfa irrigated by flooding) and the hope for the place. There, Oscar and three other ejidatarios (holder of a share in common lands) have opted to see things different; they have, in simple greenhouses, vegetable cactus irrigated by drip. Entering the greenhouse immediately gives you not only another temperature but an odor of organic matter and fertile soil. It is a true pleasure to see the cactuses big and healthy. By showing the journalists the difference between an abandoned, and non-nutrient, white and salty parcel’s soil (the alfalfa absorbs too much water and nutrients leaving the soil bald and with nothing to offer) with the halophytic pasture (interesting and heterogenic in texture and color) and with the one at the cactus’ greenhouse (black and rich in organic matter), is quite evident which one is the desirable agricultural practice to let to your children!!! And not to say saving the most old and interesting ecosystem in the world…

At night I had dinner with Omar Vidal and the WWF team. They informed me that neither the governor of Coahuila, the SEMARNT’ secretary nor the water comission secretary (CNA) were coming because some ejidatarios were paid by ‘obscure forces’ (Jose Barrios, former manager of Coahuila’s CNA is my bet) to make a riot. 
On Friday February 1, Luis Fueyo and Englantina Canales arrived anyway to make from the Mezquites River a beautiful scenery for the international wetlands day. Omar, Englantina and Luis spoke marvelously. The ejidatarios that are opting to save Ciénegas were awarded, and the 5 rioters with banners were heard…the argument was incredible!!! I want to keep irrigating: as I want to, what I want to, and because I want to!!!     
By bringing Luis Fueyo and Englantina Canales with their people to a fishless Churince with less water than ever, on February, the argument of doing whatever you want, whenever you want to, sounded truly criminal.
It’s a good thing that tomorrow comes into force the ban, and that the users of the wells and rainfall runoff will have to get in line. Who says, [maybe] by praying for several storms, and by applying the law seriously this paradise still can be saved.

Wednesday December 5, 2012

I can’t believe it!! The last action of Felipe Calderon on November 30 was to sign the Cuatro Ciénegas-Ocampo-Lamadrid ban, and put it in the official diary!!! Check it out.


It is not a perfect decree, but is perfectible and most of all, any legislation is MUCH better than none.
We have to maintain the surveillance over what the new national water comission (CNA) is going to do about it; mostly, we have to cross our fingers so Luis Fueyo continues as the commissioner of protected areas, without a doubt we own this accomplishment to his administration.
On the other hand,   what is needed with real urgency is a consciousness shift from both the water users and the responsible of making the law to be fulfilled. If this does not occur, no ban is worth, because for example, In El Hundido there is a ban and Florentino Rivera Jr.  fails to comply it, and no one says anything…
Who says, maybe the change announced by the Mayas is precisely this, a change in our perception and in our conscience, to think more in the common good than in small selfish victories. I still believe in miracles.


Friday, November 4, 2011

Desert Dessert in Natural History


By Valeria Souza

If you are interested in getting the original image or the PDF, please contact Valeria

Churince is Basically Dead

Churince System Status aquatic fauna. by Evan Carson, August 20, 2011

Water Levels

Degradation of the system has proceeded rapidly since the February-March 2011 trip. The most obvious change is profound drying of the lower third to half of the system (Table 1). In addition, there has been complete drying of the large Northeast Marsh (represented by CHU06 of Carson, 2005), which, prior to this year, has never occurred in recorded history from 1998- present. The closely associated CHU05 was also dry and the peripheral habitat of CHU07 was reduced to two remnant pools. These latter two sites are peripheral habitats of Laguna Intermedia and formerly received direct flow from the Northeast Marsh (CHU06). Similar to the Northeast Marsh (CHU06), the lower system peripheral site CHU12 (Carson, 2005) was completely dry, which has also never occurred in recorded history from 1998- present. Although water was maintained in the nearby, downstream pool of CHU13, water level was declining rapidly (several cm in a week) downstream at CHU 14 and the final downstream pool (within the Rio Churince) at site CHU15 was almost completely desiccated. Laguna Grande and most of the lower Rio Churince, aside from small, isolated pools, was completely dry. Remaining hydrated stretches of the Rio Churince had little to no flow in the lower system.

From CHU11 to the headspring at CHU01, water was present, but the levels were lower than in February-March and declining. In particular, Laguna Intermedia was substantially lower than in February-March (10 cm reduction). This in part reflects normal changes in seasonal balances of spring-flow and evaporation rates, but the water levels were visibly lower than previously experienced by this researcher. The upper system was essentially stagnant, with much reduced flow from normal conditions and consequent development of flocculent surface mats, alga blooms in the rio, and significant reduction of breeding and feeding habitat.

Physicochemistry

No records of physicochemistry were obtained in Feb-Mar. 2011, but ones obtained in May 2011 demonstrate the severity of change in the system from its normal state. Specific conductance (a proxy for salinity) in the headspring was increased by ~10 % relative to pre-crash levels recorded by this researcher on numerous trips in winter, spring, and summer from 1998-2003. Below the headspring, salinity increases throughout most of the system, including all of the Rio Churince, were 50% or greater relative to pre-crash levels.Increased salinity potentially results from reduced of input of freshwater from the deep aquifer, reductions in lower salinity, shallower parts of the aquifer (tapped first by irrigation efforts), lower pressure and longer transport time to the headspring (in effect increase time available to saturate water with salts prior to emergence at the headspring), or some combination of these factors.

Changes in Faunal Distributions and Abundances

For the three snail species for which long-term records are available (Mexipyrgus churinceanus, Mexithauma quadripaludium, andNymphophilus minckleyi), abundances have been dramatically reduced so as to be virtually absent. Only Nymphophilus minckleyiwas observed alive on this trip, despite considerable effort to capture all three species from the headspring and the Rio Churince, and N. minckleyi and M. churinceanus from Laguna Intermedia.Mexipyrgus and Mexithauma appear functionally extirpated from the system, though N. minckleyi appears to be maintaining a small but viable population. The latter is the most hardy of the three species, so this is not surprising.

As in Feb-Mar. 2011, numbers of the endemic cichlid, Herichthys minckleyi, appear reduced in Poza Churince and the upper Rio Churince. What remains unknown is the composition of the feeding morphs. With high snail abundance in the past but few present today, it is predicted that the molluscivore form of H. minckleyi has or will experience significant population reduction or elimination. Abundances of the detritivorous and piscivorous forms are unknown, with the latter perhaps reduced due to competition with the introduced jewel cichlid Hemichromis guttatus. Hemichromis guttatus remains abundant in much of the remaining Rio Churince system, and is highly abundant in Poza Churince. Abundance of C. bifasciatus in Poza Churince is much reduced from the time prior to system degradation. The Mexican tetra Astyanax mexicanus andendemic Gambusia longispinis appear to have been extirpated from the system altogether. The distribution and abundance of Cyprinella xanthicara* and Dionda sp. have been sharply reduced since Feb-Mar 2011, as there is little typical habitat remaining in lower reaches of the system.

*In October 2011, no C. xanthicara were observed despite considerable effort to verify persistence. It is possible the species remains present at a critically low abundance for the time being, at least.

More environmentally hardy species have also been reduced in population size and shifted in geographic distribution in the system.Cyprinodon atrorus has been forced to move upstream into areas formerly occupied by other species, as lower reaches have dried and upper reach habitats have changed to become more environmentally typical of habitats preferred by this species. Nonetheless, the majority of this species habitat has dried and population sizes have declined sharply. Similarly, the abundance and distribution of Cyprinodon bifasciatus has been significantly reduced, such that the species is apparently restricted to Poza Churince. Consequent to the changes in abundance and distribution of C. atrorus and C. bifasciatus, the hybrid zone between these species has essentially disappeared because the two species no longer overlap extensively in range and number in the middle-lower system.

It is critical that water levels be monitored and change quantified. Also, it is urgent to verify that water extraction in the Hundido Valley has indeed ceased. The Becerra canal needs to be taken out of operation or, at a minimum, severely reduced in flow capacity. Finally, water levels and physicochemistry must begin being monitored in Becerra, Juan Santos, and the headwaters of the Rio Mesquites in order to identify sudden or beginning changes in the viability of these systems. Degradation of the system has accelerated since the May 2011 trip. The most obvious change is reduction and fragmentation of Laguna Intermedia. This system is now very shallow and consists of several rapidly desiccating pools. The most well developed stromatolite fields in the Churince system are now exposed and dry. Downstream of Laguna Intermedia, the Rio Churince has become a series of stagnant, desiccating pools down to CHU11 of Carson (2005). For all purposes, reaches below Laguna Intermedia are lost, and Laguna Intermedia itself is in ecological collapse. Upstream of Laguna Intermedia, the system remains largely stagnant, with much reduced fish and other faunal populations. Algal development and reduction of feeding and breeding habitat is severe in the upper Rio Churince. Poza Churince appears lower than in May, but this could be due to seasonal increases in evaporation rate.

It appears faunal extirpations in this system are occurring and increasing in extent. The endemic Gambusia longispinis is almost certainly extirpated from this system and the native Mexican tetra (Astyanax mexicanus) appears extirpated, as well. Hydrobiid snail endemics, particularly Mexipyrgus and Mexithauma, are close to or already are extirpated from this system. Unfortunately, Poza Churince is the type locality for Mexipyrgus churinceanus. Concerns are also growing that Cyprinella xanthicara has been lost form the system or is close to that point. However, most species are in great danger of being lost from this system, unless higher water flows return very soon. Although Cyprinodon atrorus is well adapted to live in severe habitats, most of the habitats typical of this species are now completely dry. Cyprinodon bifasciatus has suffered drastic population size reductions in its typical habitat (Poza Churince, Upper Rio Churince) and is now absent from Laguna Intermedia. Peripheral habitats in the upper Rio Churince system still maintainDionda sp., Gambusia marshi, Cyprinodon atrorus, Cyprinodon bifasciatus, and Cyprinodon hybrids. As such, these areas are oddly ideal for testing hypotheses of ancient stabilization of hybrid lineages in the basin that arose after regional drying over the last 10,000 or more years.

At this point, the Churince system is essentially a dead system, at least relative to its former condition. It is imperative to return Poza de la Becerra to a vibrant and healthy spring, and revive its associated marsh system and most importantly its outflow, the Rio Garabatal. It is also critical to end unsustainable water extraction in the Hundido Valley, which likely means closing all existing wells and ending water extraction in that basin. I have observed change in Churince of the last 13 years and have an intimate knowledge of the history of Cuatro Ciénegas. Without immediate restoration of Becerra/Rio Garabatal and closure of the wells in the Hundido Valley, the Churince system will almost certainly dry completely within a few years at most. The entire western basin is likely to follow, and when and if this extends the to the headwaters of the Rio Mesquites, the basin will have lost the vast majority of its unique habitats and species, as well as the economic opportunity for its people. Arresting water extraction may already be too little, too late.

Contact information:

Evan W. Carson

Dept. of Biology

University of New Mexico,

MSC03 2020

1 University of New Mexico Way

Albuquerque, New Mexico, 87131-0001

Email: evan.carson@gmail.com or carson1@unm.edu

Website: http://www.evanwcarson.com

Friday, October 28, 2011

see nature news blog

http://blogs.nature.com/news/2011/10/mexican_ponds_shrinking_faster.html

Friday, October 21, 2011

In Cuatrociénegas Coahuila, water is everything



by Valeria Souza



Cuatro Ciénegas is an oasis in the desert a “unique time capsule.” that it hosts ancient forms of marine microbial life that have evolved and persisted over the past 3.5 billion years. The only one of its kind, the microbial community of the basin provides a unique window on what the earliest life on Earth was like.



While pursuing my research, we also discovered that large portions of Cuatro Ciénegas were going dry since October 2002, due to the pumping of underground aquifers to irrigate alfalfa farms in the region. Many species in its rich ecosystem -- which include rare native fish, turtles, snails, and shrimp, in addition to the marine microbial community -- were disappearing due to the lack of water.


Having witnessed these changes, I began raising awareness about the unique value of the basin and the threat to it posed by the pumping. Her efforts quickly grew into a conservation effort that involved farmers, ranchers, researchers, villagers, and international NGOs. In the beginning of 2003 we produced molecular ecology data to demonstrate the connectivity of the deep aquifer between the protected area of Cuatro Cienegas and two neighboring valleys were intensive pumping was occurring. I got the attention of part of the federal government, but never knew who I was against, since those deep wells were authorized illegally by the water lord in Mexico (Comision Nacional de Agua, CNA) a shareholder of the powerful dairy industry LALA and belonged to the governor of the state of Coahuila another shareholder of LALA. In 2004 the fight for Cuatro Cienegas started to get public attention at the national level and CNA invested several million pesos to take my credibility down. However, my communication skill had improved by then and the science we produced got the respect of town-people, journalists, the public politicians and NGOs who were watching our movements quietly from backstage. In 2006 Coahuila got a new governor who was not an ally of the dairy industry and joined the fight proclaiming that Cuatro Cienegas was a state treasure.

In 2007 apparently we won an impossible battle, the voluntary withdraw of LALA ranch, the regulation of the water pumping in one of the 3 valleys and congress budget to change the way water was extracted in Cuatro Cienegas by the ejidatarios, poor ranchers that also grew alfalfa. LALA also end up paying the environmental education and the breakfast of the rancher kids. This was a battle of principles, I said NO on devastation in 2002 because as a scientist I had the crystal ball to predict the outcome of the pumping, and as a mother I had to show my children that a sustainable future was something worth fighting for. Without doubt Cuatro Cienegas gave me an unexpected power, the power to convince. On retrospective, it was not so hard because I was fearless, persisted and learned to communicate to the public the scientific evidence.



At the core is a vision of sustainability for the entire ecosystem, including its human inhabitants. However, between 2007 and 2011, the happiness of winning an impossible battle slowly dissolved and was replaced by sobering despair. The money of the state of Coahuila and the federation (1000 million pesos) that was going to be used to change the irrigation system was starting to get used on doing the exact opposite, draining the water out of the ecosystem faster than it can be replaced. My favorite ecosystem in Cuatro Cienegas, the Churince, died in October 2011. Clearly, to believe in politicians was not a good idea, that is why Science for the People is a way to put a very high value on the water, a value that the water users can see and benefit from.

I want to transform Cuatro Ciénegas from a sleepy water hole surrounded by poverty and ecological damage to a place with sustainable agriculture, eco-centered tourism, a cutting-edge research center serving all ages, from young children to graduate students, and a science museum explaining why those beautiful and unexpected blue pozas with stromatolite reefs escaped becoming fossil sepulchers, and how the creatures in them survived all the geological catastrophes that created Mexico.




In order to transform a poor endangered site to a rich conserved paradaise, we have developed a way of working by which research and public engagement reinforce each other. Through this approach, which we call "science for the people," the team has helped farmers and ranchers understand the economic impacts to their livelihoods caused by the ongoing irrigation. They have also worked with the community to find ways of supporting the local economy while also ensuring the sustainability of the basin. One strategy they developed is to commercialize genes(1) that are found in the basin and might be useful to biotechnology. Profits from the patents are returned to the local people.

(1)http://www.scidev.net/en/news/commercialising-genes-may-help-conserve-mexican-oasis-.html


However, a survey by our team in October showed widespread losses of species in a summer drought during which pumping continued. In response we are ralling the community to hold the government accountable for its commitments. They have started an international petition list that has today 2500 signature (2) and are exploring other means as well. Valeria also started a blog(3), that we hope will help keep the issue in the public eye.


(2) http://www.thepetitionsite.com/takeaction/116/895/434/

(3) http://souzacuatrocienegas.blogspot.com/


This time, I hope that the Cuatro Cienegas ancient creatures win this final battle for survival, they can not afford anymore setbacks. When I saw this October the fish carcasses carpeting the dry sediment of an ancient lagoon and the desperate scratches of the last marine turtles looking for water, I got terribly sad and weep, but soon this was replaced by active anger. These dead could be prevented if action was done, as promised, in 2007. “This is not “déjà vu”, by now I have the power of a fulfilled prediction. This time, the ball is rolling much faster and the national response to the turtles cry is not waiting to see what will happen to the rest.

Our vision of sustainable development in Cuatro Cienegas is obviously focused on science, Cuatro Cienegas can become not only the national hub for biotechnology and organic hydroponic agriculture but also a sanctuary were tourists come to marvel after understanding that this oasis can explain us all why this planet is blue and so full of life.





--
Dra. Valeria Souza Saldivar
Instituto de ecología UNAM
Ap 70-275, CU, Coyoacan,
Mexico DF, 04510, México

Churince's Laguna Intermedia